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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in several tumors, which leads to chromosome instability, and therefore might contribute to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial position in marketing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been described. Two more splice variants have been claimed but specific nucleotide sequence data is just not nevertheless available.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized through the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make certain the best possible success are...
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This guideline outlines further feasible optimization of cellular and immunoassay parameters to make certain the best possible final results are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is precise.
Focus on Facts Cyclin E belongs on the hugely conserved cyclin loved ones, whose associates are characterized by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which contribute to your temporal coordination of every mitotic occasion. Cyclin E forms a fancy with and functions as a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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